I understand there are a number of protein-protein interactions, but what types of interactions exist? and what are the characteristics of them?
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1$\begingroup$ Characteristics? They happen between proteins. $\endgroup$– Konrad RudolphMar 12, 2012 at 23:41
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$\begingroup$ characteristics of the interaction type, i.e, what makes a hydrophobic interaction? $\endgroup$– harpalssMar 13, 2012 at 0:20
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$\begingroup$ I would suggest asking "what are the various types of protein-protein interactions" $\endgroup$– bobthejoeMar 13, 2012 at 7:33
1 Answer
There are so many types of protein-protein interactions via various domains, such as SH2 binding (in RTK signaling), Pleckstrin Homology domain (involved in signaling) among others. This site gives a nice list: http://pawsonlab.mshri.on.ca/index.php?option=com_content&task=view&id=30&Itemid=63
Of course, protein-protein interactions rely on the premises of basic biochemistry:
- van-der waals interactions (at the most basic level)
- Electrostatic interactions
- "Lock and Key" model: Some proteins have specific binding pockets for domains of other proteins
- Induced fit
There are many ways to study protein-protein interactions, including, but not limited to
- Pull-down assays
- 2D SDS-PAGE / MS
- Yeast two-hybrid
- Immunoprecipitation
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$\begingroup$ jp has a good point - technically there is no 'hydrophobic force' but when computations are done, a hydrophobic term is often made in the calculations. $\endgroup$– shigetaMar 13, 2012 at 4:10
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$\begingroup$ Although hydrophobicity isn't a "force", shouldn't it still be included in the answer (creates an entropic advantage)? $\endgroup$ Aug 17, 2012 at 2:22