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what is the best way to eliminate clumping suspension cells used for transfection. Anti clumping agents interfere transfection and hence can't be used. Though maintaining the cell density low helps, but doesn't help in the long run.

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changing the media can help - for example, chemically-defined media designed specifically for protein expression might work better than media containing serum, which can promote the expression of cell adhesion proteins –  MattDMo Sep 13 '13 at 14:20
    
Thanks for the reply. I am currently using invitrogen's CHO S SFM which, though not completely defined chemically, is serum free. –  Vishwanath Maralingannavar Sep 18 '13 at 16:14
    
I had clumping issues with HepG2 cells for a long time, I had to split them every 2 days and trypsanize them for 30-45 minutes at 37C to really break them up. Then I would split them at 2 or 3 to 1 ratios so that they formed a more uniform distribution. Less density seemed to cause more clumping, probably because each cell grew into a clump. These aren't CHO cells, but might be helpful. –  user137 Jul 27 at 6:16

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