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what is the best way to eliminate clumping suspension cells used for transfection. Anti clumping agents interfere transfection and hence can't be used. Though maintaining the cell density low helps, but doesn't help in the long run.

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changing the media can help - for example, chemically-defined media designed specifically for protein expression might work better than media containing serum, which can promote the expression of cell adhesion proteins –  MattDMo Sep 13 '13 at 14:20
    
Thanks for the reply. I am currently using invitrogen's CHO S SFM which, though not completely defined chemically, is serum free. –  Vishwanath Maralingannavar Sep 18 '13 at 16:14
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