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I want to do the above assay but by mass spectrophotometry (MS). Hence I need to optimize in MS friendly buffers like formic acid, ammonium formate, ammonium acetate. Has any one done this before...please reply with procedure. What would be the optomum pH for the Buffers?

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I'm having difficulty imagining an ELISA using MS. Would the MS be used to detect the product of the linked enzyme? –  Alan Boyd Oct 23 '13 at 17:48
    
YES. MS will be used to monitor –  dutta Oct 23 '13 at 18:18
    
@dutta how would you monitor the ELISA? They really are not very compatible. How are you planning to process the samples? Is the idea that you would do the ELISA first, then take the positive samples and try to get a MS hit? –  Atl LED Oct 24 '13 at 0:06

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