I've done O- and N- linked enzymatic deglycosylation of purified and lyophilized proteins. Following some modifications to the manufacture's protocol, I had great results.
I now want a student to deglycosylate some total cell lysates (TCL) of infected cells. Can he just Vacufuge/vac-spin Triton-X TCL's and then proceed with the deglycosylation? The first step is often resuspend in water. I'm wondering if ether the salts would need be removed by dialysis, or if it doesn't matter, if he can start just from the lysate.
The antibodies we have for western detection are excellent, so that is unlikely to be a limiting factor.