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I would like to know your experiences with SuperScript® VILO™ Master Mix. According to de manufacturer, this kit maintains linearity from 1 pg to 2.5 µg in a 20 µL reaction.

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What are your experiences? I have to amplify some genes I found interesting using RNA-seq.

Thanks, Bernardo

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closed as too broad by Chris, Bez, GriffinEvo, Chris Stronks, user137 Dec 8 at 23:45

There are either too many possible answers, or good answers would be too long for this format. Please add details to narrow the answer set or to isolate an issue that can be answered in a few paragraphs.If this question can be reworded to fit the rules in the help center, please edit the question.

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