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I am writing an assay about HbA affinity in one specific bird species. In the paper I've read mixing technique is used to determine the P50 values. Can someone explain me this technique and what is tonometered blood?

P50 values of fresh whole blood were determined with the mixing technique using tonometered blood (Scheid and Meyer, 1978)

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I think this is the paper in question. –  Atl LED Nov 7 '13 at 20:47

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