My daughter (10th grade) is doing a science fair project on the toxicity of triclosan for the algae Selenastrum capricornutum. She is wondering the best way to measure the effect, given the limited resources at the school.
- Should she use the school’s UV/Vis spectrophotometer or just do low-tech cell counting?
- If she uses the UV/Vis, does she have to do a calibration curve where she manually counts the cells for some number of data points?
- Or can she just compare %absorbance to the controls and before/after numbers?
- Or is it already know for this particular algae at a particular wavelength that a certain %absorbance equals a certain number of cells?
- If she uses the UV/Vis, does that indicate the total number of cells, dead or alive, or does it only measure live cells?
The trial is over 96 hours. Thanks!