Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Join them; it only takes a minute:

Sign up
Here's how it works:
  1. Anybody can ask a question
  2. Anybody can answer
  3. The best answers are voted up and rise to the top

I'm working with luciferase and I want to be able to take a photo of it. The trouble is, I can see the luciferase glowing in all of its glory in front of me but no matter how hard I try, I can't take a photo of the luciferase with my DSLR (Nikon D80).

I'm curious if I'm missing a certain lens or if I should be shooting using a different lighting setup. I'm already exposing for 30". Perhaps longer?

share|improve this question
You might want to look at too – Rory M Mar 7 '12 at 8:49
That I have done – bobthejoe Mar 7 '12 at 14:47
Seem to have got some quite thorough answers there :) – Rory M Mar 7 '12 at 19:02
up vote 4 down vote accepted

You'll also want to make sure that you are imaging in complete darkness because you may have very low photon emission from your luciferase system and any amount of background light can overwhelm your signal with noise.

share|improve this answer

You are looking for a bioluminescence imaging device. These have very sensitive CCD camera and exposure times are around 5 minutes in complete darkness.

share|improve this answer
here's an example: – Amy Mar 7 '12 at 17:50

Your Answer


By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.