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If I do a transduction with a P1 lysate prepared from a strain that is pro*C*::Tn10 and the recipient is wild type, will all my recombinants be ProC- and Tet resistant since the Tn10 is in the proC gene?

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Slightly confused by this - what are you going to plate on after the transduction step? –  Alan Boyd Nov 18 '13 at 9:18

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