The typical procedure to obtain cDNA from genomic extracted DNA is the following:
RNA is extracted from desired tissue, RNA/DNA hybrid is obtained by virtue of reverse transcriptase RNA dependent DNA polymerase, H RNAse creates gaps in RNA of DNA/RNA hybrid and with the aid of a typical DNA polymerase dsDNA is transcribed, right?
Then, the newly synthesized cDNA is linear (like a "small chromosome")? How is it protected from the nucleolytic activity? Is the procedure really like I described or does the reverse transcriptase already give a dsDNA from single stranded mRNA?