I've seen in MIQE guidelines that calibration curves a needed when performing RT-qPCR. I'll use genomic DNA to perform these calibration curves.
My question is that I don't know the range of concentrations to test.
The genes I will check for differential expression have logFC between 2-8 (base 2 log).
50 ng/μL is the sample concentration I will have when performing the Comparative CT Experiment, but 98% is ribosomal RNA.
Please let me know you suggestions and possible reference books/papers to revise.
Thanks for you help. Best regards, Bernardo