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I began reading this paper (http://www.biomedcentral.com/1471-2105/14/56) and had a few questions about mass spectrometry terminology that I couldn't find answers to elsewhere. Consider the following line:

"The resulting peptide signals in the LC-MS map are referred to as features while the selection of features for fragmentation with MS/MS is called precursor ion selection."

I'm not sure what an LC-MS map is, so I can't properly understand what features or precursor ion selection are: can someone define these terms for me with examples?

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LC-MS stands for Liquid chromatography–mass spectrometry. The LC-MS map shows the separation of samples by polarity and mass. –  Mys_721tx Jan 8 '14 at 6:21
As I understand precursor ion refers to the tryptic peptide ions prior to fragmentation after the first round of MS. –  WYSIWYG Jan 8 '14 at 9:13

1 Answer 1

LC-MS map they refer to is the projection of two separation method (Liquid Chromatography - Mass Spectroscopy) results into an imaginary plane. It's a conceptual map. From this map, peptide signals referred to as "features" (article uses this term) are selected for the next level of fragmentation and then MS again.

Figure 1 of the article you cite simulates a small fraction of this larger map.

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