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So, some people and I encountered an adventure game where you have a bee in a jar and need to move it from place to place before it suffocates, and someone tried to find out how long a bee would actually survive in the jar. We've found three relevant papers Oxygen consumption in the foraging honeybee depends on the reward rate at the food source, Oxygen consumption and body temperature of active and resting honeybees. and Physiological Correlates of Foraging Efforts in Honey-Bees: Oxygen Consumption and Nectar Load, which has given us what we think is a good high-end guess of 40 microliters of oxygen per minute consumed.

However, we don't know at what point the oxygen level would get too low for the bee, or if carbon dioxide poisoning would occur. Does anyone have a source for the minimum oxygen levels a bee needs to survive, or the point at which CO2 toxicity becomes a problem?

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visit this link you get some ideas dude google.co.in/… –  gopinath Jan 15 at 12:22
    
@gopinath First thing they talk about is air holes. –  Canageek Jan 15 at 18:50
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As an amateur beekeeper, I think I can work up an answer for you in the next few days. The answer will be regarding the typical honeybee. Is that acceptable? Just to be sure, the question you want answered is "What is the minimum level of oxygen for a bee to survive?" On a side note, I think it is a horrible game if it requires that a creature suffocate to determine a winner. –  fredsbend Jan 15 at 20:35
    
@fredsbend In the game you have to transport a bee from place to place in a sealed jar, to protect it from a toxic atmosphere. However, the bee runs out of oxygen and dies very, very quickly, limiting where you can take it. We thought this was unrealistic, and wanted to work out what what the actual time was. –  Canageek Jan 15 at 21:48
    
@Canageek Well, I was going to mention that oxygen may not be the limiting factor. Bees are quite susceptible to high temperatures, and in a sealed jar there is no ventilation to cool the bee. Put the jar in the sun and that bee will die in under 15 minutes. The average bee's body temperature cannot stand anything much hotter than 110 for longer than a minute or two. –  fredsbend Jan 15 at 21:56
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