Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

I found this sentence in my notes interesting

E-NOS and n-NOS are Ca-calmodulin dependent ferments. They are synthesized in small concentration as a response to physiological signals which increases the level of Ca2+ in cell.

Does it mean that e-NOS and n-NOS are synthesized by mediation of Ca and calmodulin?

I remember how RNS can be derived from nitric oxide and superoxide.
Are e-NOS and n-NOS expressed on macrophages after induction by cytokines and microbial products, notably interferon-gamma and lipopolysaccharide?

share|improve this question
    
What is the reference for your notes? Can you cite a valid reference? –  WYSIWYG Dec 5 at 9:42
    
Sorry, I cannot. This is a classroom discussion, mostly based on current textbooks in Physiology and Pathophysiology. Good bet would be in Robbins. –  Masi Dec 5 at 14:26

1 Answer 1

up vote 2 down vote accepted

Does it mean that e-NOS and n-NOS are synthesized by mediation of Ca and calmodulin?

Calmodulin is activated by binding to calcium and NOS is activated by binding to Ca2+-calmodulin. The binding changes the structural conformation which renders the enzyme active. See this.

Are e-NOS and n-NOS expressed on macrophages after induction [...]

Macrophages generally express iNOS upon induction by cytokines. The activity of iNOS is shown to be different in humans compared to mice; humans have low activity [ref]. However mouse macrophages have been shown to express eNOS constitutively and eNOS-knockout mice show reduced NFkB production and iNOS induction.

share|improve this answer

Your Answer

 
discard

By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.