When penicillin-resistant bacteria are grown in liquid culture media, B -lactamase is secreted into the medium. The supernatant of such a medium can be assayed for B- lactamase activity. Culture supernatants different organisms , which are suspected to be penicillin-resistant, were obtained and were assayed for B-lactamase activity. The corresponding absorbance value were measured at a certain wavelength.
Let us assume that supernatant from organism 1's culture had absorbance value X and 2 had absorbance value Y.
My question : If X>Y, does that mean that more B-lactamase was being produced by organism 1 ?