When penicillin-resistant bacteria are grown in liquid culture media, β-lactamase is secreted into the medium. The supernatant of such a medium can be assayed for β-lactamase activity. Culture supernatants different organisms , which are suspected to be penicillin-resistant, were obtained and were assayed for β-lactamase activity. The corresponding absorbance value were measured at a certain wavelength.
Let us assume that supernatant from organism 1's culture had absorbance value X and 2 had absorbance value Y.
My question : If X>Y, does that mean that more β-lactamase was being produced by organism 1 ?