I have a list of differentially expressed genes from an RNA-Seq experiment in Xenopus laevis that I'm looking to functionally annotate with GO-terms. As X.laevis is not listed in DAVID it seems I have 2 options:
- BLAST my DE genes against a database with GO terms (human/mouse) to look for orthologs
- Use a program that assigns GO from BLAST results, such as Blast2GO
I've been trying with Blast2GO recently, but find if very slow and generally lacking - does anyone have any experience doing GO in Xenopus or other non-model organisms?