Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

I am currently working with a peptide which is an analogue for glp-1, but during invitro studies am not able to detect for the presence of GLP1- receptors. The cell line used is Min-6. How do I detect it?

share|improve this question
If you have an antibody against the GLP-1 receptor, you could make a whole cell lysate and do a western. How old are your cells? According to this they alter their behaviour over time and alter the expression of genes. –  Chris Feb 13 at 14:30
Try polyclonal antibodies. It might just be that your antibody isn't picking up the epitope. –  dayuloli Feb 14 at 5:43
add comment

Your Answer


By posting your answer, you agree to the privacy policy and terms of service.

Browse other questions tagged or ask your own question.