Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

Lets imagine that I have mammalian cells that I've immobilized on a filter. Now I want to keep providing small molecules to these immobilized cells without resolubilizing the cells.

The caveat is that I would like to do so without fixing the cells or waiting for them to adhere to the filter. The small molecules are small enough to diffuse through the filter.

share|improve this question
perhaps you can dip the filter plate in a medium containing the small molecules.. and then keep changing the bath after few days.. btw you want a batch or continuous system?? –  WYSIWYG Jun 10 '13 at 7:55
@WYSIWYG, Batch should be fine. The question is what would be the setup to do so. Would it be as simple as reversing a 96 well lid over and placing the filter plate on top? What about the wetting? –  bobthejoe Jun 10 '13 at 22:46
I am not very sure but you may try the plates used for growing ES cells over feeder layers separated by a membrane –  WYSIWYG Jun 11 '13 at 4:38

1 Answer 1

Perhaps you're looking for these hanging inserts, for example: "Millicells"

example of inserts

share|improve this answer

Your Answer


By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.