Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

Lets imagine that I have mammalian cells that I've immobilized on a filter. Now I want to keep providing small molecules to these immobilized cells without resolubilizing the cells.

The caveat is that I would like to do so without fixing the cells or waiting for them to adhere to the filter. The small molecules are small enough to diffuse through the filter.

share|improve this question
    
perhaps you can dip the filter plate in a medium containing the small molecules.. and then keep changing the bath after few days.. btw you want a batch or continuous system?? –  WYSIWYG Jun 10 '13 at 7:55
    
@WYSIWYG, Batch should be fine. The question is what would be the setup to do so. Would it be as simple as reversing a 96 well lid over and placing the filter plate on top? What about the wetting? –  bobthejoe Jun 10 '13 at 22:46
    
I am not very sure but you may try the plates used for growing ES cells over feeder layers separated by a membrane –  WYSIWYG Jun 11 '13 at 4:38
add comment

Your Answer

 
discard

By posting your answer, you agree to the privacy policy and terms of service.

Browse other questions tagged or ask your own question.