I was wondering if anyone had recommendations for good, large (hopefully 100kDa+), control proteins that would be present in most mammalian cells. I'm working mostly with tissue samples from humans and mice, and airway epithelium in particular if that matters. So far the best I've thought of is HSP90, but that is far from ideal for a number of reasons. My target proteins are between 125 and 210 kDa. To improve resolution, we've run pretty much everything under 90kDa off the gel.
That removes the old standbys like actin or GAPDH. I understand that the protein levels could vary between cell types for a good loading control target. Bonus points if you know a good antibody clone to it.