Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

I heard this fact that you can use some [hypertriade] for vibrio cholera diagnosis which has compontents

  • sucrose (yellow)
  • mannose (yellow)
  • arabinose (do not ferment; stay dark pink)

I did not find this hypertriade agar on Google. It reminds me of Egg Yolk Agar, but I think cannot be it because of the arabinose. Otherwise, it seems to be similar.

What are the best detection medias for cholera?

share|improve this question
1  
I don't see how you could have a single medium that would simultaneously test for fermentation/non-fermentation of three different sugars. From the colours that you mention this is clearly based on a tetrazolium salt, but if all three sugars were present then two of them would be fermented and you would get a conversion to a yellow product, regardless of the inability to ferment arabinose. –  Alan Boyd Feb 22 at 12:32
    
@AlanBoyd I added an explanation what the hypertriad may refer to: there can be three selective media in one petri dish. I am not sure why they are using the word hypertriade or hypertriad. They seem to be using only sucrose, mannose and arabinose. Is there any collective name for such combination? –  Masi Feb 22 at 14:51
1  
Is there a reason you wouldn't be using TCBS? Why the "hypertriade" in the first place? If you're designing a specimen screen, you might use 4 instead. I also, I've never heard the phrase hypertriade, and DDG/Google failed to teach me. Do you have a reference for that term? –  Atl LED Feb 23 at 2:29
    
@AtlLED Make that an answer and I will accept it. I do not know why the assistant was using the word hypertriade. I have no idea of the origin. –  Masi Feb 23 at 8:01
add comment

1 Answer 1

up vote 0 down vote accepted

You should use Thiosulfate-citrate-bile salts-sucrose agar for the detection of all vibros, including cholera. When cholera is presant, you should expect round, largish (not huge), yellow colonies. This is the CDC recommended diagnoses method.

Again this question might be better phrased "what are the best detection medias for cholera," or "what plate screens should be conducted when vibros are suspected?"

share|improve this answer
add comment

Your Answer

 
discard

By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.