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I've reading about proteomics and how it can be used with mass spectrometry to sequence digested peptides.

Reading this paper the author states (in box 2):

peptide fragmentation induced by collisions with residual gas, and bond breakage mainly occurs through the lowest energy pathways - that is, cleavage of the amide bonds

Why is cleavage predominately at the amide bond? What makes the amide bond the lowest energy pathway?

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closed as off-topic by WYSIWYG, Bez, Chris, fileunderwater, GriffinEvo Dec 8 at 16:34

This question appears to be off-topic. The users who voted to close gave this specific reason:

  • "General chemistry questions are off-topic here, but can be asked on Chemistry Stack Exchange." – WYSIWYG, Bez, Chris, fileunderwater, GriffinEvo
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This question is probably better suited to Chemistry.SE –  MattDMo Mar 11 at 20:49
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@MattDMo While this is a chemistry question, it is also about biologically important molecules, so it is still on-topic here. –  Mad Scientist Mar 11 at 21:34
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@MadScientist Even though it is about a biological relevant molecule the principle behind this question is chemistry. Note that this is not about peptide formation and degradation in-vivo; it is about collision induced decay (nothing biological here). –  WYSIWYG Dec 8 at 12:29

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