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I have 2 transgenic lines of Drosophila. I would like to cross them and get both transgene in the same fly. To start this cross, does it matter which fly is female or male? Or as long as the female is virgin, it does not matter?

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flies aren't my field of expertise, but you probably would want to verify by PCR that any crosses were homozygous in any case. –  shigeta Mar 12 at 14:18
    
Ah no, I have certain markers for it. So thats not a big problem. I am asking a really silly question probably. –  golgicik Mar 12 at 18:46

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As long as none of your genes are X-linked and you use virgins it shouldn't matter and you'd get the same proportion of double homozygotes. You may want to perform a reciprocal cross (https://en.wikipedia.org/wiki/Reciprocal_cross) if you don't know what chromosome these genes are on (though flybase.org is useful too).

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Thank a lot! It helped. –  golgicik Mar 13 at 21:04

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