What does single channel microarray mean? When is it used? Thank you for your case in advance.
Single channel refers to the system used to detect the labelled probes. A single channel array, as shown in this slidedeck (slides 6-8) uses one type of molecule for detection. In the example, (slide 8) the optical label is phycoerythrin. Contrast that to a double channel array (e.g slide 7) that uses a "green" or "red" molecule used to label probes. Detecting two different labels requires two channels, one to detect red and one the the green. The key to using two (or more) labeling molecules is to ensure that your detection system can discriminate between the light spectrum emitted by each of the labels. Many modern microarray readers have designs that handle multiple labels.