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New to molecular and have learned to design primers from google/youtube so any info would help

Would someone be willing to share their protocol for degenerate primer design?

Breakdown: Trying to design degenerate primers to amplify/clone specific GPCR genes from an unsequenced genome of a particular fish.

I'm using Ensembl and Genbank for sequences of closely related fish but the complete gene has only been sequenced in 2 other fish species. I use ClustalOmega for alignment. Is it okay to just use 2 other species for generating degenerates?

1) are there any other good databases for gene sequences?

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