Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

When transcription factors attach to the DNA strand - How do they know in which direction they have to initialize the transcription by rna polymerase? Is it always read in the same direction anyway? What about those 'promoters'? Do they influence the transcription direction?

share|improve this question
    
Do you mean by direction which of the two DNA strands is transcribed? Or do you mean whether the polymerase goes from 5' to 3', or from 3' to 5' on a single strand? –  Mad Scientist Apr 22 at 16:12
    
@MadScientist Although the first question is also interesting, the second one is the one which I am interested in at the moment. –  Sprechblaseningenieur Apr 22 at 18:45

1 Answer 1

up vote 3 down vote accepted

Transcription always proceeds in the direction 5' (5-prime) to 3' (3-prime) on the coding strand of DNA. Binding of both transcription factors and RNA polymerase to DNA depends on sequence motifs in the DNA. Transcription always happens in the same direction with respect to the chemical structure of the coding DNA strand, while the transcription direction with respect to the structure of the chromosome depends on which strand the coding sequence is found on (and thus which strand is defined as the coding strand for that gene).

share|improve this answer
1  
Just to clarify, RNAP moves relative to the template strand from 3' to 5'. The transcript is polymerized from 5' to 3' and has the same sequence as the coding strand (except U instead of T). –  canadianer Apr 22 at 18:52
    
Thanks, good point. The whole process is described, with figures, in an NCBI online book here: ncbi.nlm.nih.gov/books/NBK22085 –  jarlemag Apr 22 at 19:10
    
dudes! thanks! that helped a lot!!! –  Sprechblaseningenieur Apr 22 at 19:50
    
Glad to hear that. You can also communicate that an answer is useful by clicking the checkmark next to the answer, "accepting" it. –  jarlemag Apr 23 at 0:11
    
I don't think transcription always goes $5' \rightarrow 3'$ wrt to coding strand. See en.wikipedia.org/wiki/… –  Superbest Jun 20 at 11:49

Your Answer

 
discard

By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.