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I'm working with murine B-cells. The general protocol is to keep cells on ice to keep them from dying but I've noticed that it makes these cells aggregate and precipitate out. I've heard suggestions that these cells should just be kept at room temperature. How would I be able to determine which conditions at which I should be keeping my cells?

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I know I'm not supposed to ask for clarification here, but I think it's completely necessary in order to provide an appropriate answer. Could you please clarify what you're doing with your cells? i.e. do you need to keep them physiologically active? How long are they out of the incubator? If cells are just going to FACS, ice is fine, just pulse the pellet prior to reading, a little EDTA can also help keeping them from clumping. In general, I would recommend ice or 37 degrees most of the time. –  Dr. T Jul 30 '13 at 21:56
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