in vitro compartmentalization (IVC) is one of those technologies that everyone knows about, talks about, but never actual does due to the rather technical difficulties in setting the system up. I was wonder if anyone not named Tawfik, Griffiths or someone who did a post-doc with them have been successful in making IVC work? Details in how stable emulsions and a homogeneous mixture of cell-free components in the droplet are made would be wonderful.
It is actually surprisingly easy and reproducible to make emulsions with defined components. We do it routinely in our lab: http://www.sciencemag.org/content/332/6026/209.full - CBT protocols http://www.sciencemag.org/content/336/6079/341.full - CST protocols