Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Join them; it only takes a minute:

Sign up
Here's how it works:
  1. Anybody can ask a question
  2. Anybody can answer
  3. The best answers are voted up and rise to the top

Routinely, lysis of cells with hypotonic solutions is carried out along with some physical (douncing) or enzymatic (lysozyme) method to break open the cells. If one were not to do any of these and just resuspend cells in water, how long would it take to lyse the cells? I was discussing with a lab mate who claimed it would take hours while in my understanding, salt equilibrium should not take very long (maybe on the order of minutes?) and hence, lysis should be done within 20-30 minutes.

share|improve this question
this is more of an anecdotal answer but I put sheep blood cells in pure water in a lab that I use in my organismal bio course and they lyse almost instantly. I have not timed it but I would say less than a minute. – KennyPeanuts Apr 17 '12 at 22:53

In the case of red blood cells, hemolysis in pure water occurs at just over 1 minute, 30 seconds, according to this paper. (Osmotic Properties of the Erythrocyte, M.H. Jacobs and Arthur K. Parpart, The Biological Bulletin, 1932)

share|improve this answer

Your Answer


By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.