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I'm trying to get a sense of the variety of methods used for determining the number of cell surface antigens and receptors. This is notably different from determining the affinity of these surface markers.

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Are you looking for extraction techniques, or techniques to analyze your extracted molecules? – CHM Apr 21 '12 at 0:01
up vote 4 down vote accepted

Do you mean to count numbers of both antigens and receptors in live cell?

Possible ways:

  1. FRET (unlikely to work)
  2. imaging of receptors with single molecule resolution
  3. western blot to roughly estimate amount of receptors
  4. single cell proteomics techniques (not very suitable)
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FRET could work. He'd have to tag both the antigen and antibody, though He probably would be better off with standard fluorescence spectroscopy. – CHM Apr 21 '12 at 3:13

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