Tell me more ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.
up vote 2 down vote favorite

I'm trying to get a sense of the variety of methods used for determining the number of cell surface antigens and receptors. This is notably different from determining the affinity of these surface markers.

share|improve this question
Are you looking for extraction techniques, or techniques to analyze your extracted molecules? – CHM Apr 21 '12 at 0:01

1 Answer

up vote 4 down vote accepted

Do you mean to count numbers of both antigens and receptors in live cell?

Possible ways:

  1. FRET (unlikely to work)
  2. imaging of receptors with single molecule resolution
  3. western blot to roughly estimate amount of receptors
  4. single cell proteomics techniques (not very suitable)
share|improve this answer
FRET could work. He'd have to tag both the antigen and antibody, though He probably would be better off with standard fluorescence spectroscopy. – CHM Apr 21 '12 at 3:13

Your Answer

 
discard

By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.