Ischemia is roughly defined as insufficient blood flow to a specific region of the body and in the literature it is generally considered as causing oxygen and glucose deprivation in the tissue and therefore cells. Can we induce ischemia by depriving cells from oxygen and glucose in the medium or does it require more than that and ischemia is more complicated?
edit: i know the definition of ischemia maybe i should have said 'mimick' instead of 'induce'. Anyway, here is the better version of my question:
Ischemia is defined as blood flow shortage in a tissue and it is one of the main causes of non-apoptotic cell death. In an article i was reading, the authors claim that they induced ischemia in the glucose-serum containing medium. Does this mean they deprived the cells from oxygen and thus mimicked ischemia? Is this the way to create ischemia in a cell culture? What other ways can there be? Should not cells also be deprived from glucose?