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I'm looking for a software to calculate Fst from 3 loci DNA data of individuals from a metapopulation. I don't have any prior on the population structure (have no idea of the number of subpopulations for example)

I've been looking at FSTAT but it doesn't run on MAC.

Do you have any easy alternative?

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it should be pretty easily implementable in perl or python. – WYSIWYG Jun 26 '14 at 6:39

If You are familiar with R, try package adegenet. It contains very nice tutorials explaining its usage, including the very R basics. If You require graphical interface, install RStudio - very nice front-end for R. There are more possibilities how to calculate Fst in R, but adegenet is one of the most powerful genetic packages there.

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