Does anybody have any estimate on the number of RNA pol II molecules in the nucleus of a given human cell? For example, how many RNA polymerase type II protein complexes would there be on average at a given time in, say, a human immortalized lymphoblastoid cell line (LCL)?
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it is highly variable even in a single cell type. Dont know for a human cell but this is the range for ecoli: 1500-11400 check this site out. got this number from there (its quite useful for questions like these): http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101440&ver=3&trm=RNA%20polymerase You can estimate protein copy numbers and there are techniques available for doing so. One of such techniques: http://www.mcponline.org/content/11/3/O111.009613.long You can also calculate concentration using traditional techniques like western blot and elisa and calculate approximate copy-numbers (you have to also calculate average cell volume and number of cells for protein extraction. |
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