I have a microbiology question.
When we put bacterial cells in sucrose solution with concentration higher than 0.5M we observe plasmolysis - the cytoplasmic membrane detaches from the cell wall due to water loss in order to balance the osmotic gradient.
If after that we put some lysozyme in the solution, the cells become spherical as they turn into spheroplasts, but they are still alive.
If we put lysozyme in a solution that contains bacterial cells, but no sucrose, they die. So my question is how does sucrose protect cells from lysis?