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for a simulation I am developing I would like to know how the actin network in neurites is distributed.

Is actin confined to the periphery or is the whole neurite shaft containing actin with a rather constant density?

If you have any papers backing up the one or the other view don't hesitate to pass the link.

Thanks for answering.

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does this help? (neuraldevelopment.com/content/pdf/1749-8104-6-27.pdf) –  Bez Jul 22 at 17:48
    
on first sight this paper only gives a 2D distribution of actin over the whole neuron or? I would like to know what you would see if you cut a neurite and subsequently look "into" the cross section. Is the actin only found on the outer part of the cross section or evenly distributed across the circle? –  user3683367 Jul 22 at 18:01
    
Just look at papers doing confocal microscopy/imaging of actin with z-stacking (z refers to the axis) since thats the main approach people use for 3D imaging and people also use delta vision microscope with z-stacking and deconvolution. –  Bez Jul 22 at 18:13

1 Answer 1

up vote 0 down vote accepted

Actin is required for the neurite architecture and is present throughout the shaft (along the direction of the neurite process)

See these images.


                                        enter image description here

                                        [Source]


   enter image description here

                                             [Source]

These are not from research papers but the sources are trustworthy.

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From those images I would not deduce that the neurite shaft has a substantial actin skeleton but rather a small amount of "misguided" filaments. Is that right? –  user3683367 Nov 22 at 17:39
    
Why would you assume some misguided filaments? The region indicated as meshwork is essential for the structure. –  WYSIWYG Nov 23 at 15:21
    
In the second picture actin and tubulin are labeled. I cannot see any actin along the shaft except of growth cone areas. So there is no substantial amount of actin concentrated along the shaft. Or? –  user3683367 Nov 25 at 11:28
    
OK I didnt understand what you meant by shaft. I thought you were referring to the filopodial region. Yes actin is not abundant in the axonal regions –  WYSIWYG Nov 25 at 11:31

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