We have a long protocol that we are optimizing that includes DpnI digestion of a PCR product (to remove any of the template DNA if it's methylated, and while we're not certain in the blind tests, likely relevant DNA will be methylated).
I'm wondering if we can extend the DpnI incubation/reaction time to 4-5 hours. This will make concurrent steps in the screen easier to manage. Has anyone extended DpnI digestions? How "leaky" or nonspecific of a process can it be?