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In preparing agar plates with NGM, and also liquid cultures for growing c elegans, how essential is the addition of cholesterol and heavy metals? I have prepared both. I can't notice any obvious difference and I hate having to add all these supplements.

I'm using yeast extract and peptone as the primary components of the base.

Isn't this just supposed to be a moist semi-soft substrate for them to crawl around, their nutrients come from the bacteria they eat.

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1 Answer 1

up vote 6 down vote accepted

C. elegans needs cholesterol, but can't make it. Since bacteria don't make cholesterol either, the food bacteria aren't a source. As explained in the linked paper, lack of cholesterol affects normal development.

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And without supplementing there is enough in the peptone and yeast extract to already. I wonder if this stuff is only essential for cholesterol biosynthesis studies? –  rhill45 Aug 30 at 21:02
    
Just FYI @alan Boyd's I upvoted and would like to keep open for a few days to see what else out there but this is prob best foreseeable response –  rhill45 Aug 30 at 21:05

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