In preparing agar plates with NGM, and also liquid cultures for growing c elegans, how essential is the addition of cholesterol and heavy metals? I have prepared both. I can't notice any obvious difference and I hate having to add all these supplements.
I'm using yeast extract and peptone as the primary components of the base.
Isn't this just supposed to be a moist semi-soft substrate for them to crawl around, their nutrients come from the bacteria they eat.