Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

Bovine serum albumin, Avidin, Ficoll-70 and Dextran-70,are they positively charged or negatively charged ? And which other solvents can be used as a substitute to water for preparing solutions in each case ?

share|improve this question

closed as unclear what you're asking by Superbest, Rory M May 12 at 20:56

Please clarify your specific problem or add additional details to highlight exactly what you need. As it's currently written, it’s hard to tell exactly what you're asking. See the How to Ask page for help clarifying this question.If this question can be reworded to fit the rules in the help center, please edit the question.

2  
What part is giving you difficulty. At least for the charges you can use Gooooooooooogle... –  nico Jun 1 '12 at 6:53
    
yes but mainly I require to know about the solvents. –  Stp30 Jun 4 '12 at 4:28
    
Flagging because this is a bunch of unrelated questions. (each molecule should be its own question, solvents are also a separate question entirely) The question as is is unlikely to be useful for future users. –  Superbest May 12 at 18:42

1 Answer 1

For charge, you could check this one by sequence level, for example for Bovine Albumin (Uniprot: P02769) you can get the theoretical pI of 5.82, as the mean of pKa aminoacid values, to do this can use protparm, so when the pH > pI, the protein has a net negative charge and when the pH < pI, the protein has a net positive charge.

And there are only two organic polar solvents I known to work proteins, DMSO (pKa = 35) and Dimethylformamide (DMF, pKa=-0.01 (20ºC))

share|improve this answer

Not the answer you're looking for? Browse other questions tagged or ask your own question.