Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Join them; it only takes a minute:

Sign up
Here's how it works:
  1. Anybody can ask a question
  2. Anybody can answer
  3. The best answers are voted up and rise to the top

Many protocols suggest that beta-mercaptoethanol is necessary for growing cells. It is a reducing agent but what does it mechanistically do for your cells. When would one not add it.

share|improve this question
up vote 2 down vote accepted

2-me is a reducing agent necessary to be added to help keep free radical oxygen from affecting mouse cells. It is generally not necessary for human cells.

Also from S Bannai and Ishii et al., 2-mercaptoethanol improves tumor cell uptake of cystine by creating a reducing environment.

share|improve this answer
    
BTW, the statement about human cells is not true. The addition of bME to primary immune cell cultures, especially B and T cell cultures, is common. – MattDMo Dec 2 '15 at 23:39
    
Common doesn't necessarily mean necessary. – bobthejoe Dec 29 '15 at 18:01

Your Answer

 
discard

By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.