Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

My questions are:

  • What techniques are available for doing so?

  • Are they destructive ?

  • What is their accuracy ?

share|improve this question
add comment

1 Answer 1

Again, not an expert, but I found this article seems to say it pretty well:

Telomere's are most commonly measured by qPCR of the repetitive regions with degenerate oligomer sequences such as "TTAGGG and CCCTAA repeats" Although articles like this one appear to advance measurements in vertebrates, this reference from 2011 implies two techniques continue to be most common:

1) qPCR with the appropriate oligos and then running them on an agarose gel

2) Southern blot of a restriction digest that leaves the telomeres intact

Clearly sequencing techniques don't do well with repetitive sequences such as this, so in general this still seems like the most common method.

This would indeed be quite destructive, at least for the cells used, though a biopsy would be enough, though apparently micrograms of DNA are required. Kind of a large amount.

The accuracy would then be limited to that of the gel or blot. That would generally be a percentage of total length, I think 2% might be obtainable, but I'm not sure about that part.

share|improve this answer
add comment

Your Answer

 
discard

By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.