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What is the appropriate method to pipetting up and down mammalians cells to sufficiently break up clumps. This should also keep in mind the issues from Pipetting damage on cells. Visually one can see some clumps but even after pipetting for long times they don't break up.

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Is triteration a real word? At first I assumed you meant trituration, then I googled for it and effectively I got some "pipetting-related" hit. Still it sounds a bit "fishy", as it is only a very few results (plus it is not in the dictionary). –  nico Jun 26 '12 at 6:36
    
@nico, Trituration does seem to fit the bill. It seems more physorganic chem than biology but we are creating a homogeneous mixture by shearing. I was hesitant about changing it since it was so fishy. –  bobthejoe Jun 26 '12 at 16:07
    
Have you tried using trypsin? It's definitely more of an art than a science, as it affects different cells in different ways, but it can be very useful for detaching cells from the dish and each other. –  MattDMo Dec 1 '12 at 14:07

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