Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

What is the appropriate method to pipetting up and down mammalians cells to sufficiently break up clumps. This should also keep in mind the issues from Pipetting damage on cells. Visually one can see some clumps but even after pipetting for long times they don't break up.

share|improve this question

closed as too broad by Chris, WYSIWYG, Bez, Chris Stronks, GriffinEvo Dec 5 at 11:49

There are either too many possible answers, or good answers would be too long for this format. Please add details to narrow the answer set or to isolate an issue that can be answered in a few paragraphs.If this question can be reworded to fit the rules in the help center, please edit the question.

Is triteration a real word? At first I assumed you meant trituration, then I googled for it and effectively I got some "pipetting-related" hit. Still it sounds a bit "fishy", as it is only a very few results (plus it is not in the dictionary). –  nico Jun 26 '12 at 6:36
@nico, Trituration does seem to fit the bill. It seems more physorganic chem than biology but we are creating a homogeneous mixture by shearing. I was hesitant about changing it since it was so fishy. –  bobthejoe Jun 26 '12 at 16:07
Have you tried using trypsin? It's definitely more of an art than a science, as it affects different cells in different ways, but it can be very useful for detaching cells from the dish and each other. –  MattDMo Dec 1 '12 at 14:07

Browse other questions tagged or ask your own question.