Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

I want to measure alkaline phosphatase activity using PNPP in my mutant bacteria strains, but all the protocols I found involve purification of the phosphatase (which I have no need of).

Does anyone know a good protocol which involves using only the supernatant, without purification of the enzyme? What is the preferred buffer for the reaction?

share|improve this question
add comment

2 Answers 2

I found this kit that appears to be compatible with biological samples. Rather than buying the kit you may also be able to imitate / modify their protocol to do the assay with your super natant.

share|improve this answer
    
Thank you, but I don't think I can imitate the kit - they don't share the components of the buffers. It did give me some idea of how the protocol is done, so thank you. –  Innab Dec 24 '11 at 7:46
add comment

Your Answer

 
discard

By posting your answer, you agree to the privacy policy and terms of service.

Not the answer you're looking for? Browse other questions tagged or ask your own question.