Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

I'm beginning to do some cell-binding assays and I would like for my proteins to not be endocytosed by my mammalian cells. Typical suggestions are for the cells to be kept on ice and that the binding experiment also occurs when the cells are chilled.

However, to be sure it has been suggested that I use endocytosis inhbitors. So my questions are, when are the endocytosis inhibitors necessary? How would I know that endocytosis inhibitors are necessary? Which ones would I actually use?

I recognize that there are both clatherin and non-clatherin mechanisms of internalization. Which mechanism would be more useful to study for an antibody-receptor interaction?

share|improve this question

closed as off-topic by Chris, GriffinEvo, Chris Stronks, anongoodnurse, Cornelius Dec 5 at 15:12

This question appears to be off-topic. The users who voted to close gave this specific reason:

  • "Homework questions are off-topic on Biology unless you have shown your attempt at an answer. For more information see our homework policy." – Chris, GriffinEvo, Cornelius
If this question can be reworded to fit the rules in the help center, please edit the question.