I have E. coli with transformed plasmid on agar in a vial. It has been stored at -20 degree C without glycerol stock for 18 hours.
This is a continuation of: Survival of E. coli on agar plate at -20 degree C without glycerol stock
I have transfered this vial from -20 degrees to 4 degrees after 18 hours when I determined that I had made a mistake. Since this is a very important vial and I don't have another one, what should I do in this stage?
What is the procedure for saving the plasmid if I am unable to save the whole cell?