I'm trying to find a good protocol for plasmid minipreps and I'm looking at 3 preps I've found:
- Using phenol/chloroform
- extract with phenol:chloroform:isoamylalcohol,
- isopropanol precipitation, 12,000g spin down,
- rinse with cold 70% ethanol.
- Using lysozyme
- lyse with lysozyme,
- remove pellet,
- isopropanol precipitation,
- wash with cold 80% ethanol.
- Using alkaline lysis
- open cells with 80% glucose in EDTA buffer,
- add SDS and NaOH,
- pellet protein/membrane with acetic acid/acetate,
- ispropanol precipitation,
- wash with cold 70% ethanol.
They all differ in how to break open the cells and separate plasmids from the rest of the cell -- quite a bit. Can anyone help me figure out which protocol is best here?