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I faced with a non-coding transcript that specified as one the isoform of BIN1.

It sounds that this isoform generated as a result of alternative splicing with a intron retained; am I right? However, Ensembl reports that the transcript has 5 exons, could you please let me know more about this event? How does the transcript have 5 exons and all are non-coding? Also, I'll glad to hear why the organism or cell has retained such a transcripts? they have any function? Thanks

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  • $\begingroup$ An exon is just a sequence that is retained in the mature RNA. It doesn't have to be protein coding. $\endgroup$
    – canadianer
    Sep 18, 2015 at 16:56
  • $\begingroup$ I could not find the sequence of this transcript but it must have become non coding most probably because retention of the intron must have caused a frameshift leading to loss of the ORF (there are no stop codons). $\endgroup$
    – WYSIWYG
    Sep 18, 2015 at 17:00
  • $\begingroup$ Some changes could also destabilize RNA and make the turnover so fast that it does not code. As an example, the intron could add a target for interfering RNA (siRNA) that both blocks translation and forces the degradation. $\endgroup$
    – WilliamL
    Sep 19, 2015 at 18:29

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