I've being using this lysis buffer for fungal DNA extraction,lysis buffer - 400mM TRis-HCl(pH8.0), 60mM EDTA(pH8.0), 150mM NaCl, 1% SDS and containing 40microgram/ml RnaseA.
It is keep giving very light bands on 1% agarose gel and shear appearence. After extraction I have to follow PCR and sequencing. If there are inhibitors, how can I get rid of those ?