I'm looking for some pointers to proteins that produce at really gigantic levels in E. coli and yeast (S. cerevisiae). Can anyone point to some champion proteins?
Even in inclusion bodies and non functional, just cases where the yield is very high - how much of a given protein can a liter of E. coli or S. cerevisiae make?
I've seen bands on gels like this, but I've probably not seen the champions:
It would be great if you could cite and discuss the plasmid/promoter used and whether codon optimization or other such tricks made a difference.